Biobottle Instructions and Supplies Generally you need to decide the following things before you begin construction: What kind of ecosystem do you want to create (aquatic, terrestrial, or a combination of both, look below for diagrams of these basic types)? How many bottles do you want to use? Where will you keep the biobottle? Do you want to build it in room C-8 and then take it home or do you want to do the whole project at home? What kinds of things do you think will do well in this type of environment until the end of the semester? Will you be able to provide a food source for all the organisms you put in the bottle?
	All of the following materials except the bottles are available in room C-8 for you to use. You may also look in the biobottle books for examples as well as look at the demonstration biobottle. Basic supplies needed to make a biobottle: Empty, clean, clear, plastic 2 liter soda bottle(s) Scissors, utility knife Clear, wide, tape  Silicone sealant Glue gun, glue sticks Tool for poking holes in bottles Sharpie pen Screen material
	All of the following materials are available for you in room C-8. Suggested abiotic components to add to the biobottles: Water Soil Gravel Sand
	Organisms highlighted in red are available in room C-8 the rest need to be gathered outdoors. Suggested biotic components to add to the biobottles: Insects Spiders Moss Small plants Water Plants (for aquatic biobottles) Small fish Snails Water bugs Potato bugs Seeds Worms
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